easysep human cd33 positive selection kit Search Results


cd33 pe  (Miltenyi Biotec)
95
Miltenyi Biotec cd33 pe
Cd33 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lps-ek ultrapure  (InvivoGen)
96
InvivoGen lps-ek ultrapure
Lps Ek Ultrapure, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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easysep cd33 selection kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc easysep cd33 selection kit
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Easysep Cd33 Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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easysep pe selection kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc easysep pe selection kit
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Easysep Pe Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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easysep hla chimerism positive selection kits  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc easysep hla chimerism positive selection kits
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Easysep Hla Chimerism Positive Selection Kits, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34 microbead kit  (Miltenyi Biotec)
99
Miltenyi Biotec cd34 microbead kit
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide  (Millipore)
90
Millipore propidium iodide
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Propidium Iodide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs  (Thermo Fisher)
90
Thermo Fisher pbs
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd16-fitc (mem-154  (Thermo Fisher)
90
Thermo Fisher cd16-fitc (mem-154
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Cd16 Fitc (Mem 154, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmso  (Millipore)
90
Millipore dmso
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Dmso, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh67 green fluorescent cell linker midi kit  (Millipore)
90
Millipore pkh67 green fluorescent cell linker midi kit
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Pkh67 Green Fluorescent Cell Linker Midi Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lymphoprep (stem cell)  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc lymphoprep (stem cell)
a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + <t>CD33</t> + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.
Lymphoprep (Stem Cell), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + CD33 + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.

Journal: Nature biotechnology

Article Title: Development and function of human innate immune cells in a humanized mouse model

doi: 10.1038/nbt.2858

Figure Lengend Snippet: a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + CD33 + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.

Article Snippet: Human CD33 + cells were enriched by magnetic isolation (EasySep CD33 selection kit, StemCell Technologies).

Techniques: Injection, Staining, Flow Cytometry, Expressing, Control